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Update 2003 You can read this as a PDF. Much better. Yeast and Rotenone by Richard Katz 1970
volume 12. number 3 FEBS LETTERS North-Holland Publishing Company - Amsterdam January 1971
GROWTH PHASE AND ROTENONE SENSITIVITY IN TORULOPSIS UTILIS:
DIFFERENCE BETWEEN EXPONENTIAL AND STATIONARY PHASE
Want to Skip to the graph of what I found as shown in fig. 1 ?
(Note from 1999: The followup article
Note from 2003: The followup article in pdf form, so you can even read it! Yeast Rotenone Site I and above all Oxygen Supply (variously known as Oxygen Concentration or Oxygen Tension) 1971
Katz R., Kilpatrick L., Chance B. (1971). Acquisition and loss of rotenone sensitivity in Torulopsis utilis. Eur. J. Biochem. 21, 301-307.
was the first mention that Candida (=Torulopsis) yeasts have site 1 energy conservation = site 1 oxidative phosphorylation = site 1 of the electron transport chain in stationary phase, but not in exponential phase = log phase of growth. )
As the years have gone by, it strikes me that nobody really cares much about rotenone acquisition, or its loss; or even the acquisition of Site I; even though modern gene expression techniques could elucidate this and find out something very interesting about stationary phase and a number of other things. But even moreso, it strikes me that nobody cares much about whether or not their cultures of microorganisms have enough oxygen. We're talking zero available oxygen here.
The Johnson Research Foundation Medical School. University of Pennsylvania, Philadelphia, Pennsylvania
Received 17 October 1970
I - Introduction
Rotenone, the principal insecticidal constituent of derris root, has been shown to inhibit respiration and electron transport in isolated mitochondria from rat liver [ 1]. beef heart , pigeon heart , insect flight muscle , as well as from a number of other sources. It has recently been shown to Inhibit the oxidation of low potential flavoprotein, associated with the oxidation of NADH and of pyruvate and a-ketoglutarate dehydrogenases  . Rotenone has no detectable effect on the activity of the electron transport system in Saccharomyces yeasts [6, 7]; rotenone insensitivity has been referred to as constitutive in these organisms .
Rotenone sensitivity in Toruloplis utilis yeast, (and some other yeasts), however, has been the subject of much investigation and controversy. Ohnishi et al  reported in 1966 that T utilis grown in batch culture was'sensitive'to rotenone. Light et al.  reported in 1968 that respiration of T. utilis cultured in a chemostat on a glycerol-limited medium was inhibited 30-70% by rotenone; however if the chemostat was switched from glycerol-carbon-limited growth to iron-limited growth cells were obtained in which respiration was completely insensitive to rotenone. Ohnishi  reported in 1970 that analogous results could be obtained with batch cultures of T. utilis. She found that if the initial iron concentration in the culture was 1.1 µM Fe or more the cells produced were sensitive to Piericidin A (a compound identical to rotenone in its effect on electron transport [ 11, 12]). but if the initial iron concentration was less than 1.1µM the cells were insensitive. Recently DeMaille et al  reported data supporting this effect of iron limited growth.
The present report presents rather different data. demonstrating that T. utilis is insensitive to rotenone during exponential growth in a batch culture (when iron concentration is obviously not limiting). When growth stops and the cells enter the stationary phase (in this case due to carbon limitation), rotenone sensitivity appears after a short lag.
2. Materials and methods
T. utilis was cultured at 30deg C on the synthetic medium of Galzy and Slonimski , the only modifications being increase of lron concentration to 50µM FeCI3, a tripling of copper concentration and omission of manganese. The carbon and energy source was ethanol. The culture vessel used was a 1,0 liter cylinder, approximately 5 X 50 cm, equipped with a sparger, stirrer, And Teflon-covered oxygen electrode (Yellow Springs lnstr. Co.) immersed in the culture medium. The electrode output activated a solenoid vaIve when the culture oxygen concentration fell to approximately 15% saturation, giving a pulse of oxygen sufficient to raise the 02 concentration to approximately 25% saturation. With this oxystat (designed by Dr. Dieter Mayer) the rate of oxygen consumption by the culture was monitored, by recording the rate at which the oxygen concentration fell from 25 to I5%. Cells were siphoned continuously from the culture throughout the experiment and collected in a fraction collector, maintained at 3.0 deg C, at a rate of 5 ml of cells in a 12 min interval. The turbidity of the fractions was measured in a Klett colorimeter. The fractions were then Centrifuged at 1500 rprn and the pH of the supernatant culture medium determined. The cells were washed once in phosphate buffer, 50 mM, pH 6.8, and resuspended in a small volume of the same buffer. Rotenone sensitivity was assayed by measuring polarographically the percentage inhibition of ethanol-stimulated respiration by rotenone in the washed cells at room temperature.
The results of a typical experiment are shown in fig. 1, The culture was inoculated from an exponentially growing preculture of T utilis, Throughout the period of rapid growth, all samples obtained failed to be inhibited by concentrations of rotenone up to 5 mM. Approximately 25 min after growth stopped, due to ethanol limitation, tile first partial sensitivity to rotenone became evident. This sensitivity increased steadily with subsequent aliquots, attaining a maximum of between 60 and 70 percent inhibition of respiration. The same maximum inhibition could be obtained with Piericidin A at a concentration of 4.0 µM. The half-time for acquisition of rotenone sensitivity was approximately 17 min (cf fig. 1 ).
During the period of rapid growth, the pH of the culture medium declined. This decline in pH, presumably resulting from excretion of acid by the cells, ceased abruptly when cell growth (as indicated by turbidity) stopped, When cell growth stopped, there was also an abrupt fall in total culture respiratory rate, followed by one last gasp of 02 consumption. It is approximately midway through this final burst of respiration that rotenone sensitivity is first observed.
If a second addition of ethanol is made to the stationary culture, there is a rapid decrease in rotenone sensitivity. The return to insensitivity has a half-time of approximately 14min, roughly comparable to the halftime of the original induction. This second addition Of ethanol also causes an immediate 25-fold increase in rate or culture oxygen consumption, a further lowering of pH, and after some lag a further increase in culture turbidity. Further cycles of feeding and starving (not shown) produce similar cycles in rotenone sensitivity, acid secretion, 02 consumption arid culture growth,
To test the possibility that the, oberved changes in rotenone sensitivity in the samples of intact cells were not due to an altered permeability to rotenone or piericidin A, mitohondria were isolated from cells harvested during both exponential and ethanol-depleted stationary phases of growth, according to the method reported by Light et al . As shown in table 1, the Piericidin A sensitivity of the isolated mitochondria paralleled that of the cells from which they were isolated even when substrate Ievels of NADH were employed. Results with rotenone were entirely comparable (not shown).
The one notable exception was for mitochondria respiring on ethanol, in which case piericidin A and rotenone (both at high concentrations) invariably inhibited respiration. This latter result is in agreement with Balcavage and Mattoon [ 14], who showed even in the case of Saccharomyces cerevisiae (in which the intact cells are invariably insensitive to rotenone), there is an 80% inhibition of respiration by rotenone in the isolated mitochondria. These investigators localized the source of this anomaly as yeast alcobol dehydrogenase (EC 18.104.22.168.), showing that rotenone inhibits the activity of the purified enzyme by 60% or more. The most conclusive proof that rotenone does not inhibit the rotenone serisitive site on the electron transport chain of the exponential phase Torulopsis Utilis, is that even after 73% of the ethanol-stimulated respiration has been inhibited by rotenone, the respiratory rate can be restored to I00% of control by the addition of pyruvate + malate. This result conclusively demonstrates that altered cell permeability is not a tenable explanation for the differential rotenone sensiitivity found in cells harvested from the two growth phases.
These results suggcst that in the "normal" physiological state of T utilis, where "the best standard of normality is probably the cycle of the average single cell growing exponentially in constant conditions[ 14 ], electrons flow thtough a rotenone insensitive pathway, involving an NADH dehydrogenase flavoprotein similar to the one in yeast mitochondria which oxidizes externally added NADH (fpDex) [7- 1 5]. When the cells cease to grow, they switch to a different physiologial state, in this case characterized by a drop in O2 consumption and cessation of acid production. The transition to this new physiological state apparently also involves a fundamental change in the nature of the electron flow pattern in the mitochondrial membranes, a change that can occur with a half-time of 14 minutes and can be repetitively stimulated. The aquisition of rotenone sensitivity may involve: a) diversion of electron flow from the external, rotenone/insensitive NADH dehydrogenase to a preexisting, rotenone/sensitive internal dehydrogenase; b) a change in the location or confirmation of the preexisting rotenone insensitive dehydrogenase, rendering it rotenone sensitive; or c:) derepression of the biosynthesis of the rotenone sensitive internal dehydrogenase by some intermediate of the stationary phase maintenance metabolism.
The author would like to express his heartfelt gratitude to Dr Britton Chance of the Johnson Foundation for his patience, generoSity, and Intellectual stimulation; and to Dr. Miriam Fukami of the Johnson Foundation for the large part she played in the mitochondrial preparations and assays.
Want to see a followup article that appeared in European Journal of Biochemistry? The followup was the first mention that Candida (=Torulopsis) yeasts have site 1 energy conservation = site 1 oxidative phosphorylation = site 1 of the electron transport chain in stationary phase, but not in exponential phase = log phase of growth.
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